SPONTANEOUS HYBRIDIZATION IN THE LABORATORY AND GENETIC-MARKERS FOR THE IDENTIFICATION OF HYBRIDS BETWEEN 2 ATHERINID SPECIES, ODONTESTHES-BONARIENSIS (VALENCIENNES, 1835) AND PATAGONINA-HATCHERI (EIGENMANN, 1909)
Ca. Strussmann et al., SPONTANEOUS HYBRIDIZATION IN THE LABORATORY AND GENETIC-MARKERS FOR THE IDENTIFICATION OF HYBRIDS BETWEEN 2 ATHERINID SPECIES, ODONTESTHES-BONARIENSIS (VALENCIENNES, 1835) AND PATAGONINA-HATCHERI (EIGENMANN, 1909), Aquaculture research, 28(4), 1997, pp. 291-300
This study reports the occurrence of spontaneous between Odontesthes b
onariensis 1835) and Patagonina hatcheri (Eigenmann, 1909), two South
American freshwater atherinidsl while adults of both species were bein
g held in a communal laboratory tank, and describes suitable genetic m
arkers for distinction of both species and their hybrids, Combined all
ozyme and mitochondrial DNA restriction length polymorphism analyses o
f offspring from three spawnings in the communal tanks confirmed the p
resence of hybrids along with purebreds in each spawning, Hybrids of a
n O. bonariensis mother x a P, hatcheri father were produced along pur
ebred O, bonariensis in one occasion, whereas hybrids of P, hatcheri m
others x O. bonariensis fathers were produced along purebred P. hatche
ri in two occasions, Three enzyme systems (coded by loci LDH-B, PGM*,
and CK) out of 15 (20 loci) screened allowed distinction of species
and their hybrids, but the usefulness of LDH-B for field work may be
limited by intraspecific polymorphism, Eight restriction enzymes (Apa
I, Bgl II, Eco RI, Eco RV,Eco T22I, Hinc II, Hind III, and Pvn II) amo
ng 11 produced diagnostic restriction fragments applicable for species
(motherhood) distinction, The relevance of these findings for the man
agement of natural genetic resources and the aquaculture of these two
species is discussed.