Cloning of the mouse phospholipid hydroperoxide glutathione peroxidase gene

15-Lipoxygenases and phospholipid hydroperoxide glutathione peroxidases (PH -GPx) are counterparts in the metabolism of hydroperoxy lipids and a balanc ed regulation of both enzymes appears to be important for the cellular pero xide tone regulating the expression of redox sensitive genes. In contrast t o lipoxygenases the molecular biology of PH-GPx is less well investigated. In this study we cloned the PH-GPx cDNA from a mouse fibroblast cDNA librar y and the PH-GPx gene from a mouse genomic library. The gene spans approxim ately 4 kb which includes 1 kb of 5'-flanking region and consists of seven exons and six introns. The immediate promoter region does not contain a TAT A box but there are binding sites for several transcription factors which a lso occur in the porcine gene. Our investigations provide useful tools for future targeted gene disruption studies. (C) 1999 Federation of European Bi ochemical Societies.