Expression of the genes for guanyl-specific ribonucleases from Bacillus intermedius and Bacillus pumilus is regulated by the two component signal transduction system PhoP-PhoR in B-subtilis

Promoters of the genes for guanyl-specific ribonucleases, secreted by B. in termedius (binase) and B. pumilus (Rnase Bp) in phosphate deficient conditi ons, contain regions similar to appropriate consensus sequences in promoter s of the PHO regulated genes of B. subtilis. A number of genes expressed in response to phosphate starvation in B. subtilis are regulated by the two c omponent signal transduction system PhoP-PhoR. Expression of recombinant ge nes for binase and RNase Bp in B. subtilis strains with mutations in the re gulatory protein genes of the PHO regulon was studied. Their expression is strongly regulated by the regulatory proteins of the B. subtilis PHO regulo n. (C) 1999 Published by Elsevier Science B.V. All rights reserved.