The effects on the sensory perception of texture, rheology and microstructu
re of processed cheese analogue made with different milk protein bases, and
substitution of milk fat by starch or microparticulate whey protein were s
tudied. Differences in rheological properties of the products were influenc
ed by (a) the duration of storage, (b) the cohesiveness character with resp
ect to the lipid content, and (c) the compression response and spreadabilit
y characteristics with respect to lipid, storage period and / or base; the
latter two aspects peaked at 2 months and then dropped to their initial val
ues. Scanning and transmission electron microscopy revealed differences in
the microstructure of processed cheese analogues. Products made with anhydr
ous milk fat contained higher concentrations of fat globules compared with
analogues made with fat-substitutes. Electron-dense particles were evident
in all the products, which could be found between such images and the rheol
ogical properties of processed cheese analogues. The porosity of the protei
n matrix was denser in processed cheese analogues made with high-protein sk
immed milk powder.