Detection of foetal cells in maternal blood and prenatal sex determinationby in situ hybridization. Procedure verification

Authors
Chiesa, J Ferrer, C Hoffet, M Mares, P Bureau, JP
Citation
J. Chiesa et al., Detection of foetal cells in maternal blood and prenatal sex determinationby in situ hybridization. Procedure verification, GENET A-BIO, 15(2), 1999, pp. 41-45
Citations number
22
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING
ISSN journal
10503862 → ACNP
Volume
15
Issue
2
Year of publication
1999
Pages
41 - 45
Database
ISI
SICI code
1050-3862(199904)15:2<41:DOFCIM>2.0.ZU;2-W
Abstract
We describe an enrichment of foetal cells from maternal blood with a combin ation of double density gradient and Magnetic Activated Cell Sorting (MACS) of CD71, glycophorin A (GPA), CD34 and CD36 antibodies labeled cells follo wed by fluorescence in situ hybridization (FISH) with chromosome-specific D NA probes for determination of foetal sex. (C) 1999 Elsevier Science B.V. A ll rights reserved.