Selection analysis of single-stranded clones using solid-phase techniques

Authors
Vener, T Holmberg, A Lundeberg, J Uhlen, M
Citation
T. Vener et al., Selection analysis of single-stranded clones using solid-phase techniques, GENET A-BIO, 15(2), 1999, pp. 47-50
Citations number
19
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING
ISSN journal
10503862 → ACNP
Volume
15
Issue
2
Year of publication
1999
Pages
47 - 50
Database
ISI
SICI code
1050-3862(199904)15:2<47:SAOSCU>2.0.ZU;2-F
Abstract
An efficient selection analysis of single-stranded DNA fragments using subt ractive hybridization on magnetic beads and fluorescence-based fragment ana lysis is described. This approach facilitates either direct cloning of capt ured fragments or removal of common clones from cDNA or shot-gun clone libr aries. (C) 1994 Elsevier Science B.V. All rights reserved.