Phylogeny of Vicia subgenus Vicia (Fabaceae) based on analysis of RAPDs and RFLP of PCR-amplified chloroplast genes

We report the results of two methods of DNA analysis to elucidate phylogene tic relationships among 29 Vicia subgen. Vicia species in comparison with t wo species of subgenus Vicilla sect. Vicilla. The methods employed were RAP D analysis of total genomic DNA and PCR-RFLP analysis of five chloroplast g enes, rbcL, rpoB, 16S, psaA and trnK. The results of each method were simil ar and complementary, and support the current taxonomic systems of subsp. V icia. According to RAPD and PCR-RFLP analysis the Narbonensis complex can b e considered a well separated section, which may be related to section Vici a. Sections Vicia, Atossa and Wiggersia are separate, but closely related s ections. Species of the section Hypechusa form a single monophyletic sectio n, where V. lutea, V. anatolica and V. hyrcanica are quite remote from othe r species. Our results suggest that within the subgenus Vicia, V. faba is m ore closely related to V. bithynica and that these two species are most clo sely related to section Peregrinae. We found that PCR-RFLP of cp DNA provid ed more precise information concerning relationships between Vicia sections than RAPD analysis. However, RAPD analysis was more informative concerning diversity of closely related Vicia taxa, such as the variable groups, sect ion Narbonensis and V. sativa aggregate.