F. Shinozaki et Y. Mugiya, Effects of salmon calcitonin and anti-salmon calcitonin antibody on plasmacalcium concentrations in the goldfish, Carassius auratus, ICHTHYOL R, 46(1), 1999, pp. 1-6
The role of calcitonin (CT) in plasma calcium regulation was studied by the
administration of exogenous CT and anti-salmon(s) CT antibody using goldfi
sh, Carassius auratus, loaded or otherwise with calcium. CT elicited a decr
ease in plasma calcium concentrations at a dose of 10 ng/g body weight 1 h
after administration. However, no effects were observed following doses of
30 ng and 50 ng/g 1 h, nor for the three doses 3 h after administration. In
calcium-loaded fish, the effect of CT was different depending on the dosag
e of CT. Ten ng and 50 ng/g induced a decrease and an increase in plasma ca
lcium concentrations, respectively, 3 h after administration. Anti-sCT anti
body (0.02 mu g or 0.1 mu g/g) did not affect plasma calcium concentrations
. In calcium-loaded fish, neither dose of anti-sCT antibody changed plasma
calcium concentrations I h after administration. However, following a dose
of 0.1 mu g/g, plasma calcium concentrations decreased after 3 h. A positiv
e correlation between plasma calcium concentrations and the gonad somatic i
ndex (GSI) in females was no longer apparent after administration of anti-s
CT antibody. There was no relationship between plasma calcium concentration
s and GSI in control and anti-sCT antibody-treated males. These results sug
gested that CT regulates plasma calcium concentrations in different ways de
pending on the dosage with CT having a role in calcium physiology during vi
tellogenesis.