Establishment of a human hemangiosarcoma cell line (ISO-HAS)

A cell line (ISO-HAS) has been established from tumor tissue of a human hem angiosarcoma arising on the scalp by the use of conditioned medium from a m urine-phenotypic angiosarcoma cell line (ISOS-1), Cells have been cultured for more than 2 years with up to 100 passages. The cells retained endotheli al-cell properties, such as a characteristic cobblestone appearance at conf luency, contact-inhibited growth, active uptake of acetylated low-density l ipoprotein labeled with 1,1-dioctadecyl 1,3,3,3,3-tetramethyl-indocarbocyan ine perchlorate (Dil-Ac-LDL) and CD31 expression. However, they were weakly positive for von-Willebrand-factor (vWf) antigen and for binding of Ulex e uropaeus agglutinin-1 (UEA-1) lectin, and lacked tube-formation activity. T hese findings indicate that ISO-HAS is a poorly differentiated endothelial cell line. ISO-HAS cells showed accumulation of p53 protein in the nuclei, and a new-typed p53-gene point mutation was found in exon 7 at codon 240. W hen Inoculated s.c. into severe-combined-immunodeficiency (SCID) mice, the cells showed solid-tumor growth that caused death. These properties suggest that ISO-HAS is a malignant endothelial cell line with high tumorigenicity . Int. J. Cancer 81: 305-308, 1999. (C) 1999 Wiley-Liss, Inc.