Harmful effects of UVA on the structure and barrier function of engineeredhuman cutaneous tissues

Citation
M. Robert et al., Harmful effects of UVA on the structure and barrier function of engineeredhuman cutaneous tissues, INT J RAD B, 75(3), 1999, pp. 317-326
Citations number
40
Categorie Soggetti
Experimental Biology
Journal title
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
ISSN journal
09553002 → ACNP
Volume
75
Issue
3
Year of publication
1999
Pages
317 - 326
Database
ISI
SICI code
0955-3002(199903)75:3<317:HEOUOT>2.0.ZU;2-F
Abstract
Purpose: To investigate the effects of a single UVA exposure on engineered human cutaneous tissues. Materials and methods: Skin equivalents (SE) were obtained by culturing ker atinocytes on fibroblast-populated collagen gels; epidermal equivalents (EE ) were obtained by seeding keratinocytes on non-populated collagen gels. Af ter. maturation and differentiation of the epidermis, SE and EE were expose d to 50 or 100J/cm(2) UVA. Structural damage and total epidermal lipids wer e analysed and diffusion of radioactive oestradiol was monitored 24 and 72 h post-irradiation. Results: Twenty-four hours after UVA irradiation, a disorganization of the living epidermis is observed. UVA also significantly reduced the skin barri er function and led to an increase in phospholipid and in a decrease of cer amide levels. However, both the structure and thr barrier function of SE we re recovered 72h post-irradiation, thereby suggesting that an intrinsic rep air process might exist within the irradiated SE. Conclusion: This study provides a strong evidence that UVA radiation alters both the epidermal and dermal structures, the synthesis of epidermal lipid s, and the permeability of the human skin.