The chromosomes of the: two species of needlefish, Potamorraphis cf. eigenm
anni and Strongylura microps, from fresh waters of Argentina were analyzed
using C-banding and silver staining and their karyotypes a ere described fo
r the first time. The former species possessed 2n = 54 and karyotype compos
ed of three pairs metacentric to submetacentric chromosomes and 24 pairs of
subtelo to telocentric chromosomes. The NORs were situated on the the end
of the shorter arms of one middle-sized bi-armed pair of chromosomes. The l
ater species possessed 2n = 50 and karyotype composed exclusively of subtel
o to telocentric chromosomes gradually decreasing in size, The NORs were si
tuated interstitially on the third pair of chromosomes. One or two supernum
erary chromosomes a ere observed in one individual of the sample. Present d
ata indicate greater cytotaxonomic diversity among species of needlefish wh
ich might be associated with their colonization of fresh waters.