Concentrations and glucosidic conjugation patterns of isoflavones were dete
rmined in soy foods consumed by multiethnic populations in Singapore and Ha
waii. Six raw and 11 cooked food groups traditionally consumed in Singapore
and 8 food groups consumed in Hawaii were analyzed by reversed-phase high-
pressure liquid chromatography with diode array detection. Mean total isofl
avone levels varied between 35 and 7500 ppm, with the lowest values found i
n soy milk and burgers and the highest levels observed in soybean and its s
eeds and in supplements. Total isoflavone levels and conjugation patterns v
aried as a function of soybean variety, storage conditions, and food proces
sing. A large contribution to the differences in total isoflavone content b
etween food groups was due to the water content in foods and to leaching of
polar analytes into the water phase during boiling. Soy protein drinks and
traditional soy foods were found to possess very similar isoflavone amount
s considering usual serving sizes.