Bacterial lipopolysaccharide activates nuclear factor-kappa B through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes

Bacterial lipopolysaccharide (LPS)-mediated immune responses, including act ivation of monocytes, macrophages, and endothelial cells, play an important role in the pathogenesis of Gram-negative bacteria-induced sepsis syndrome . Activation of NF-kappa B is thought to be required for cytokine release f rom LPS-responsive cells, a critical step for endotoxic effects. Here we in vestigated the role and involvement of interleukin-l (IL-1) and tumor necro sis factor (TNF-alpha) signal transducer molecules in LPS signaling in huma n dermal microvessel endothelial cells (HDMEC) and THP-1 monocytic cells. L PS stimulation of HDMEC and THP-1 cells initiated an IL-l receptor-like NF- kappa B signaling cascade, In transient cotransfection experiments, dominan t negative mutants of the IL-1 signaling pathway, including MyD88, IRAK, IR AK2, and TRAF6 inhibited both IL-1- and LPS-induced NF-kappa B-luciferase a ctivity. LPS-induced NF-kappa B activation was not inhibited by a dominant negative mutant of TRAF2 that is involved in TNF signaling. LPS-induced act ivation of NF-kappa B-responsive reporter gene was not inhibited by IL-1 re ceptor antagonist. TLR2 and TLR4 were expressed on the cell surface of HDME C and THP-1 cells. These findings suggest that a signal transduction molecu le in the LPS receptor complex may belong to the IL-1 receptor/toll-like re ceptor (TLR) super family, and the LPS signaling cascade uses an analogous molecular framework for signaling as IL-I in mononuclear phagocytes and end othelial cells.