A study was undertaken to identify the chemicals responsible for septi
c and decaying vegetation odors, not only in odor reference standards
but also in natural waters. Two complementary extraction methods-close
d-loop stripping analysis and simultaneous distillation extraction-wer
e used. Chemical identification was performed by gas chromatography (G
C) with mass spectrometry, and sensory identification was accomplished
by flavor profile analysis and sensory GC. Dimethyl disulfide, dimeth
yl trisulfide, and 2-isobutyl-3-methoxypyrazine were the principal con
tributors to decaying vegetation odors, whereas dimethyl trisulfide an
d indole were strongly associated with septic odors. In addition, the
study confirmed the presence of these chemicals as well as 2-isopropyl
-3-methoxypyrazine (associated with decaying vegetation) in water supp
lies.