Rapid and sensitive analysis of disaccharide composition in heparin and heparan sulfate by reversed-phase ion-pair chromatography on a 2 mu m porous silica gel column

Authors
Toyoda, H Yamamoto, H Ogino, N Toida, T Imanari, T
Citation
H. Toyoda et al., Rapid and sensitive analysis of disaccharide composition in heparin and heparan sulfate by reversed-phase ion-pair chromatography on a 2 mu m porous silica gel column, J CHROMAT A, 830(1), 1999, pp. 197-201
Citations number
14
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A → ACNP
Volume
830
Issue
1
Year of publication
1999
Pages
197 - 201
Database
ISI
SICI code
Abstract
A rapid and sensitive method was developed for the analysis of disaccharide composition in heparin (HP) and heparan sulfate (HS) by reversed-phase ion -pair chromatography on a 2 mu m porous silica gel column. HP and HS were d igested with heparin lyase I, II and III in combination, and the produced u nsaturated disaccharides were separated within 15 min. Calibration graphs w ere linear in the range 1 ng-1 mu g with the fluorometoric post-column dete ction using 2-cyanoacetamide. (C) 1999 Elsevier Science B.V. All rights res erved.