ALTERATION OF CYTOKINES (IL-6, IL-8, IL-1 RA) AND CELL-SURFACE MARKERS (CD25, CD30, HLA-DR) FOLLOWING SURGICAL TRAUMA

Summ.: Elective surgical approaches and trauma cause changes in the pr oduction of different cytokines, an increased production of acute phas e proteins and changes in the expression of different cell surface mar kers. Methods: In a prospective study we examined the C-reactive prote in level, the production of the cytokines IL-6, IL-8 and IL-1 RA in 25 laparoscopic and 21 conventional cholecystectomies. In addition the c ell surface markers CD25 and CD30 on different cell populations and HL A-DR on monocytes were measured. Statistical analysis was made by Stud ent's-t-test and Mann-Whitney-'s rank sum test. Results: The humoral m arkers showed a more distinct increase in patients operated on convent ionally two and 24 hours after surgery, the differences between the tw o surgical approaches were significant (p < 0.01). The cell surface ma rkers CD25 and CD30 showed the same reaction. The HLA-DR expression on monocytes was significantly lower in patients operated on conventiona lly. Conclusions: Elective surgical approaches cause changes in the im mune system, which can be evaluated by the reaction of cytokines and c ell surface markers. Laparoscopic cholecystectomies cause less activat ion of the immune system than conventional operations.