Application of molecular typing methods to dermatophyte species that causeskin and nail infections

Citation
Sa. Howell et al., Application of molecular typing methods to dermatophyte species that causeskin and nail infections, J MED MICRO, 48(1), 1999, pp. 33-40
Citations number
38
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF MEDICAL MICROBIOLOGY
ISSN journal
00222615 → ACNP
Volume
48
Issue
1
Year of publication
1999
Pages
33 - 40
Database
ISI
SICI code
0022-2615(199901)48:1<33:AOMTMT>2.0.ZU;2-Y
Abstract
Typing methods utilising DNA technology were applied to a collection of Tri chophyton mentagrophytes and T. rubrum isolates from skin and nail infectio ns. The methods included restriction enzyme analysis (REA), hybridisation w ith the DNA probe poly (dG-dT), randomly amplified polymorphic DNA (RAPD) b y PCR and restriction analysis of a segment of PCR-amplified rDNA, All thes e tests successfully differentiated the species, but few intra-species diff erences were detected. REA demonstrated some isolate variation, but this wa s limited and difficult to interpret, making it unsuitable as a typing tool . RAPD demonstrated few variations amongst T. mentagrophytes and none in T. rubrum.