S. Subramanian et Pr. Adiga, Characterization and hormonal modulation of immunoreactive thiamin carrierprotein in immature rat Sertoli cells in culture, J STEROID B, 68(1-2), 1999, pp. 23-30
Citations number
23
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
Immature rat Sertoli cells synthesize and secrete a protein species which h
as immunological similarity with chicken egg thiamin carrier protein (TCP)
as assessed by immunocytochemical localization, liquid phase radioimmunoass
ay (RIA), immunoprecipitation of [S-35]-methionine incorporated newly synth
esized proteins by polyclonal antibodies (pAbs) to chicken TCP and tryptic
peptide mapping of iodinated immunoprecipitated proteins. FSH and testoster
one together bring about 4-fold induction of Sertoli cell TCP over the cont
rol levels which is inhibitable upto 75% by an aromatase inhibitor. Additio
n of optimal concentrations of exogenous estradiol-17 beta to the cultures
causes 2-fold enhancement of secretion of TCP which can significantly be in
hibited by tamoxifen: when added along with estradiol-17 beta. These result
s show that Sertoli cells produce estrogen-inducible TCP, presumably to tra
nsport the vitamin to the developing germ cells. (C) 1999 Elsevier Science
Ltd. All rights reserved.