P. Derby et al., THE VALUE OF INCLUDING BROTH CULTURES AS PART OF A ROUTINE CULTURE PROTOCOL, Journal of clinical microbiology, 35(5), 1997, pp. 1101-1102
Three hundred seventeen clinical specimens from both superficially and
deeply infected sites were prospectively examined to assess the true
value of including liquid media as part of the routine culture procedu
re. All broth cultures were subcultured after overnight incubation ont
o plate media. The isolates obtained from the broth cultures were then
compared with the isolates obtained on primary solid media. The isola
tes obtained from the broth cultures only were evaluated for clinical
relevance by review of the patients' records. Twenty-two clinically re
levant isolates were obtained from the broth cultures only, but the is
olation of these additional organisms altered patient management for o
nly two patients. It would appear from these results that the addition
al expense and time involved in culturing clinical specimens in fluid
media is unwarranted.