DEVELOPMENT OF A REVERSE-TRANSCRIPTASE PCR-ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR QUANTIFICATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA IN PLASMA - COMPARISON WITH COMMERCIAL QUANTITATIVE ASSAYS

Authors
TRABAUD MA AUDOLY G LERICHE K COTTE L RITTER J SEPETJAN M TREPO C
Citation
Ma. Trabaud et al., DEVELOPMENT OF A REVERSE-TRANSCRIPTASE PCR-ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR QUANTIFICATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA IN PLASMA - COMPARISON WITH COMMERCIAL QUANTITATIVE ASSAYS, Journal of clinical microbiology, 35(5), 1997, pp. 1251-1254
Citations number
13
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
35
Issue
5
Year of publication
1997
Pages
1251 - 1254
Database
ISI
SICI code
0095-1137(1997)35:5<1251:DOARPI>2.0.ZU;2-I
Abstract
A quantitative reverse transcriptase PCR assay with automated detectio n by nonradioactive hybridization was developed for the determination of human immunodeficiency virus (HIV) type 1 RNA levels. This assay is based on the use of an external standard curve with an internal stand ard. The accuracy of quantification was verified by comparison with re ference commercial tests, the Chiron branched-DNA and Roche AMPLICOR H IV MONITOR assays. This assay was able to quantify viremia in patients with CD4 cell numbers below and above 500/mm(3) and to quantify some HIV strains which could not be titrated by the MONITOR assay.