EVALUATION OF A LINE PROBE ASSAY KIT FOR CHARACTERIZATION OF RPOB MUTATIONS IN RIFAMPIN-RESISTANT MYCOBACTERIUM-TUBERCULOSIS ISOLATES FROM NEW-YORK-CITY

Authors
COOKSEY RC MORLOCK GP GLICKMAN S CRAWFORD JT
Citation
Rc. Cooksey et al., EVALUATION OF A LINE PROBE ASSAY KIT FOR CHARACTERIZATION OF RPOB MUTATIONS IN RIFAMPIN-RESISTANT MYCOBACTERIUM-TUBERCULOSIS ISOLATES FROM NEW-YORK-CITY, Journal of clinical microbiology, 35(5), 1997, pp. 1281-1283
Citations number
16
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
35
Issue
5
Year of publication
1997
Pages
1281 - 1283
Database
ISI
SICI code
0095-1137(1997)35:5<1281:EOALPA>2.0.ZU;2-D
Abstract
A commercial line probe assay kit (Inno-LiPA Rif.TB) for rapid identif ication of mutations in the rpoB gene associated with rifampin resista nce in Mycobacterium tuberculosis was evaluated with a collection of 5 1 rifampin-resistant strains. Nine distinct rpoB mutations were identi fied. Concordances with automated sequence results for five wild-type kit probes and four probes for specific mutations were 94.1 and 100%, respectively. Overall concordance of the line probe assay kit with phe notypic rifampin susceptibility testing results was 90.2%.