J. Galian et P. Hudson, Cytogenetic analysis of Australian tiger beetles (Coleoptera : Cicindelidae): chromosome number, sex-determining system and localization of rDNA genes, J ZOOL SYST, 37(1), 1999, pp. 1-6
Citations number
21
Categorie Soggetti
Animal Sciences
Journal title
JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH
The chromosomes of four species of Australian tiger beetle are documented.
The male meioformulae of three species of Cincindela (tribe Cicindelini) we
re determined as follow: Cicindela cardinalba Sumlin 1987. n = 10 + X-1 X-2
X3Y; C. sp. (saetigera group) and Cicindela gillesensis Hudson 1994, n = 1
1 + X1X2X3Y. The male meioformula of Megacephala whelani Sumlin 1992 (tribe
Megacephalini) is n = 12 + XY. Fluorescence in situ hybridization (FISH) w
as used to localize the 18S-28S ribosomal gene clusters on male mitotic and
meiotic chromosomes and nuclei using a polymerase chain reaction-amplified
ribosomal probe. Fluorescence in mitosis and meiotic first prophase stages
in the three Cicindela species showed that rDNA genes are in two of the fo
ur chromosomes that form the sex vesicle. In M. Whelani hybridization in mi
tosis and first metaphase stages indicate that rDNA genes are in three, med
ium- to small-sized. autosomal pairs. Silver staining of male meiotic nucle
i reveals the presence of active nucleolar organizing regions (NORs) in the
sex vesicle of the three species of Cicindela. The cytogenetic and evoluti
onary implications of these findings are discussed.