Comparison of four commercialized biochemical systems for clinical yeast identification by colour-producing reactions

Citation
A. Paugam et al., Comparison of four commercialized biochemical systems for clinical yeast identification by colour-producing reactions, MED MYCOL, 37(1), 1999, pp. 11-17
Citations number
14
Categorie Soggetti
Microbiology
Journal title
MEDICAL MYCOLOGY
ISSN journal
13693786 → ACNP
Volume
37
Issue
1
Year of publication
1999
Pages
11 - 17
Database
ISI
SICI code
1369-3786(199902)37:1<11:COFCBS>2.0.ZU;2-2
Abstract
We compared the ability of four commercially available yeast identification systems for routine laboratory hospital use: Auxacolor(R) (AUX) (Sanofi Di agnostics Pasteur, Marne-la-Coquette), Fungichrom(R) I (FUC) and Fungifast( R) I Twin (FUF) (International Microbio, Toulon), Api Candida(R) (API) (bio Merieux, Lyon). These systems are based on obtaining a biochemical profile easily defined by colorimetric reactions. We tested 202 yeasts belonging to 19 species which were included or were not included in the manufacturer's data base of the identification systems. Without extra tests, for all the o rganisms tested, after 24h of incubation, the percentage of organisms corre ctly identified was 48% for AUX, 75% for FUC, 77% for FUF and 81% for API. However, if we consider the ratio of the number of correct identifications without extra tests with the number of yeasts included in the manufacturers ' data bases (sensitivity) the results increased to 61% for AUX, 81% for FU C, 91% for FUF and 83% for API. These systems are particularly well adapted to medical use, they are simple to set up, interpret, and have very good e fficiency for the yeasts most commonly isolated in clinical specimens. The findings reported here indicate that the most favourable results were obtai ned with FUF and API systems.