Rapid extraction of fungal DNA from clinical samples for PCR amplification

Authors
Velegraki, A Kambouris, M Kostourou, A Chalevelakis, G Legakis, NJ
Citation
A. Velegraki et al., Rapid extraction of fungal DNA from clinical samples for PCR amplification, MED MYCOL, 37(1), 1999, pp. 69-73
Citations number
22
Categorie Soggetti
Microbiology
Journal title
MEDICAL MYCOLOGY
ISSN journal
13693786 → ACNP
Volume
37
Issue
1
Year of publication
1999
Pages
69 - 73
Database
ISI
SICI code
1369-3786(199902)37:1<69:REOFDF>2.0.ZU;2-D
Abstract
A hexadecyltrimethylammonium bromide (CTAB) method for isolating fungal DNA from clinical samples, suitable for PCR amplification is described. Yeast and filamentous fungi DNA from clinical samples was amplified with primers complementary to the genes coding for rRNA, amplifying a 105 bp fragment an d internal transcribed spacer primers amplifying fragments between 242 and 622 bp. The level of sensitivity was 10 +/- 5 yeast and 28 Aspergillus fumi gatus CFU ml(-1) of biological fluid.