LOW-ENERGY AND HIGH-ENERGY COLLISION-INDUCED DISSOCIATION OF PYRIDINOLINE AND DEOXYPYRIDINOLINE IONS USING FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE ELECTROSPRAY AND LIQUID SECONDARY-ION MAGNETIC-SECTOR MASS-SPECTROMETRY

The trifunctional collagen cross-link molecules pyridinoline and deoxy pyridinoline have been structurally characterised by (1) low-energy co llision-induced dissociation (CID) (E-COM approximate to 15 eV) using Fourier transform ion cyclotron resonance electrospray ionisation mass spectrometry (FT-ICR-ESI-MS), and (2) high-energy collision-induced d issociation (E-COM approximate to 400 eV) using liquid secondary-ion m ass spectrometry (LSIMS) on a four-sector mass spectrometer. Both ESI and LSIMS ionisation readily produce the naturally occurring parent ca tions, although the LSIMS ionisation method is somewhat hampered by ba ckground ions from the matrix. Low-energy or high-energy collision-ind uced dissociation produces structurally informative fragmentations. Th e observed fragmentation patterns in the low- and high-energy CID spec tra are, however, strikingly different. Both low- and high-energy CID are structurally informative and appear to be complementary the charac terisation of collagen cross-link molecules. (C) 1977 Elsevier Science B.V.