Ss. Kumar et al., Inhibition of radiation-induced DNA damage in plasmid pBR322 by chlorophyllin and possible mechanism(s) of action, MUT RES-F M, 425(1), 1999, pp. 71-79
Citations number
41
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
Naturally occurring compounds capable of protecting DNA against ionizing ra
diation and chemical mutagens have considerable potential for prevention of
mutation-based health impairment including cancer and other degenerative d
iseases. Chlorophyllin (CHL), a water-soluble derivative of chlorophyll, ha
s been examined for its ability to protect DNA against radiation induced st
rand breaks using an in vitro plasmid DNA system. Gamma-radiation, up to a
dose of 6 Gy (dose rate 1.25 Gy/min), induced a dose-dependent increase in
single-strand breaks (ssbs) in plasmid pBR322 DNA. CHL per se did not induc
e, but inhibited radiation-induced ssbs in a concentration-dependent manner
; 500 mu M giving about 90% protection. The protection afforded by CHL was
comparatively less than that of trolox, a water-soluble analogue of alpha-t
ocopherol. To elucidate the underlying mechanism(s), reaction of CHL with t
he radiation-derived hydroxyl radical ((OH)-O-.) and deoxyribose peroxyl ra
dical (ROO.) was studied by pulse radiolysis. CHL exhibited a rate constant
of 6.1 +/- 0.4 x 10(9) M-1 s(-1) with (OH)-O-. and 5.0 +/- 1.3 x 10(7) M-1
s(-1) with ROO.. To our knowledge, this is the first report providing dire
ct evidence of free radical-scavenging properties of CHL. The results showe
d that CHL, effectively protects plasmid DNA against ionizing radiation, in
an in vitro system independent of DNA repair or other cellular defense mec
hanisms. The ability of CHL to scavenge (OH)-O-. and ROO., may contribute t
o its protective effects against radiation induced DNA damage in the pBR322
system. (C) 1999 Elsevier Science B.V. All rights reserved.