Smoothing of the thermal stability of DNA duplexes by using modified nucleosides and chaotropic agents

Authors
Nguyen, HK Fournier, O Asseline, U Dupret, D Thuong, NT
Citation
Hk. Nguyen et al., Smoothing of the thermal stability of DNA duplexes by using modified nucleosides and chaotropic agents, NUCL ACID R, 27(6), 1999, pp. 1492-1498
Citations number
34
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEIC ACIDS RESEARCH
ISSN journal
03051048 → ACNP
Volume
27
Issue
6
Year of publication
1999
Pages
1492 - 1498
Database
ISI
SICI code
0305-1048(19990315)27:6<1492:SOTTSO>2.0.ZU;2-I
Abstract
The effect of alkyltrimethylammonium ions on the thermostability of natural and modified DNA duplexes has been investigated. We have shown that the us e of tetramethylammonium ions TMA(+) along with the chemical modification o f duplexes allow the fine adjustment of T-m and the possibility of obtainin g several duplex systems with varied isostabilized temperatures, some of wh ich show greater stability than those of natural DNA. This approach could b e very useful for DNA sequencing by hybridization.