Amplification of cDNA ends based on template-switching effect and step-outPCR

Authors
Matz, M Shagin, D Bogdanova, E Britanova, O Lukyanov, S Diatchenko, L Chenchik, A
Citation
M. Matz et al., Amplification of cDNA ends based on template-switching effect and step-outPCR, NUCL ACID R, 27(6), 1999, pp. 1558-1560
Citations number
6
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEIC ACIDS RESEARCH
ISSN journal
03051048 → ACNP
Volume
27
Issue
6
Year of publication
1999
Pages
1558 - 1560
Database
ISI
SICI code
0305-1048(19990315)27:6<1558:AOCEBO>2.0.ZU;2-9
Abstract
A new method for amplifying cDNA ends is described which requires only firs t-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to ampl ify flanking sequences of any cDNA species present in the sample.