Antigenic and genetic analysis of canine parvoviruses in southern Africa

Canine parvovirus (CPV) is a significant pathogen of domestic and free-rang ing carnivores all over the world. It suddenly appeared at the end of the 1 970s and mast likely emerged as a variant of the well known feline panleuko penia virus (FPV). During its adaptation to the new host, the domestic-dog, the virus has changed its antigenic profile twice giving rise! to two new antigenic types, CPV-2a and CPV-2b. These new types have replaced the origi nal type CPV-2 in the United States of America, Europe and Japan. However, no data about the prevalence of the new antigenic types on the African cont inent are available. In this study, 128 recent parvovirus isolates from South Africa and Namibia were antigenically typed with type-specific monoclonal antibodies. No orig inal CPV-2 viruses were found and its complete replacement by the new antig enic types conforms to the situation in other parts of the world. The predo minant strain found in southern Africa was CPV-2b (66 %), which differs fro m the situation in Europe and Japan where CPV-2a is the most prevalent type . Analysis of the capsid protein DNA-sequences of four selected African iso lates gave no hint of a specific African parvovirus lineage.