Callus induction, somatic embryogenesis and organogenesis in Narcissus confusus: correlation between the state of differentiation and the content of galanthamine and related alkaloids

In vitro cultures from two strains of Narcissus confusus (Amaryllidaceae) i nitiated from mature seeds were screened for their ability to produce alkal oids. Protocols for callus induction, somatic embryogenesis and organogenes is were established. The alkaloid contents were determined by HPLC. Undiffe rentiated calli produced small amounts of galanthamine, which increased wit h the degree of tissue differentiation. Scanning electron micrographs of th e cultures at different: stages of development were made. Embryogenic friab le calli were maintained in culture for more than 2 years, retaining a high regeneration capability. All regenerated plants showed normal morphologica l characteristics.