Plantlet regeneration via somatic embryogenesis in anther callus of Vitis latifolia L.

Anthers of Vitis latifolia L. (wild grape) cultured on Nitsch and Nitsch me dium supplemented with 20 mu M 2,4 -D and 9 mu M BAP produced callus after 4-6 weeks. Subculture of callus onto Nitsch and Nitsch medium containing 10 mu M NAA produced somatic embryos within 6 weeks. On growth regulator-free Nitsch and Nitsch basal medium somatic embryos converted to plantlets in 6 -8 weeks. One gram of callus produced more than 400 somatic embryos with 13 .7% being converted to complete plantlets, which were subsequently establis hed in soil. Regenerated plants were found to have mixoploid populations of cells, 2n = 38 and n= 19.