Modulation of CREB binding protein function by the promyelocytic (PML) oncoprotein suggests a role for nuclear bodies in hormone signaling

Disaggregation of the spherical nuclear bodies termed promyelocytic (PML) o ncogenic domains (PODs) is a characteristic of acute promyelocytic leukemia . Here, we demonstrate that the cAMP enhancer binding protein (CREB)-bindin g protein (CBP) associates with PML in vitro and is recruited to the PODs i ll vivo. Through its association with CBP, wild-type PML dramatically stimu lates nuclear receptor transcriptional activity. These results demonstrate that a fraction of CBP is compartmentalized to the POD through its associat ion with PML and thus suggest that PML and other POD-associated proteins ma y play an unexpectedly broad role in aspects of transcriptional regulation and human disease.