L. Gaudreau et al., Transcriptional activation by artificial recruitment in yeast is influenced by promoter architecture and downstream sequences, P NAS US, 96(6), 1999, pp. 2668-2673
Citations number
33
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
The idea that recruitment of the transcriptional machinery to It promoter s
uffices far gene activation is based partly on the results of "artificial r
ecruitment" experiments performed in vivo.;Artificial recruitment can be ef
fected by a "nonclassical'' activator comprising a DNA-binding domain fused
to a component of the transcriptional machinery, Here we show that activat
ion by artificial recruitment in yeast can be sensitive to any of three fac
tors: position of the activator-binding elements, sequence of the promoter.
and coding sequences downstream of the promoter. Ln contrast, classical ac
tivators worked efficiently at all promoters tested. In all cases the "arti
ficial recruitment" fusions synergized well with classical activators. A cl
assical activator evidently differs from a nonclassical activator in that t
he former can touch multiple sites on the transcriptional machinery, and we
propose that that difference accounts for the broader spectrum of activity
of the typical classical activator. A similar conclusion is reached from s
tudies in mammalian cells in the accompanying paper.