INTERPLAY BETWEEN NUCLEOSOMES AND TRANSCRIPTION FACTORS AT THE YEAST PHO5 PROMOTER

Authors
Citation
J. Svaren et W. Horz, INTERPLAY BETWEEN NUCLEOSOMES AND TRANSCRIPTION FACTORS AT THE YEAST PHO5 PROMOTER, Seminars in cell biology, 6(4), 1995, pp. 177-183
Citations number
52
Categorie Soggetti
Cell Biology
Journal title
ISSN journal
10434682
Volume
6
Issue
4
Year of publication
1995
Pages
177 - 183
Database
ISI
SICI code
1043-4682(1995)6:4<177:IBNATF>2.0.ZU;2-B
Abstract
In this review, we summarize experiments which have used the yeast PHO 5 gene to determine the functional consequences of nucleosome structur e in the promoter region. In the PHO5 system, nucleosomes participate in promoter repression by interfering with factor binding. Therefore, disruption of nucleosome structure is likely a prerequisite for promot er activation. There still remain several important questions regardin g the assembly and disassembly of chromatin repression. Recent experim ents have shown that the PHO5 chromatin transition is replication and transcription independent. Nucleosome disruption does, however, depend upon binding of a transactivator, Pho4, to the PHO5 promoter. Moreove r, the activation domain of Pho4 appears to play a critical rob in chr omatin disruption.