ION-PAIR RP-HPLC SEPARATION OF THYMOPOIETIN FRAGMENTS AND RELATED PEPTIDES

Reversed phase HPLC methods have been developed for the separation of related peptides from two synthetic thymopoietin fragments: thymotrina n (Arg-Lys-Asp) and thymocartin (Arg-Lys-Asp-Val), as experimental imm unostimulant drugs. The optimization of the method using different oct adecylsilica columns, acidic buffer and hexanesulphonate as the ion-pa iring reagent has led to a method which is suitable to solve delicate problems such as the separation of diastereomeric and isopeptide deriv atives. Preliminary experiments have shown that similar results can be obtained using perchlorate as the ion-pairing reagent. (C) 1997 Elsev ier Science B.V.