SEPARATION OF A CASEIN HYDROLYZATE BY HPSEC WITH A NEW MOBILE-PHASE AND CHARACTERIZATION OF PEPTIDES BY FABMS

A mobile phase which was firstly developed for the separation of a pep tic hydrolysate from bovine blood haemoglobin by size-exclusion chroma tography (SEC) has been modified for its application to a casein hydro lysate phosphorylated by optimizing nonideal size-exclusion behavior. Peptidic fractions obtained by high performance size-exclusion chromat ography (HPSEC) were further separated by reversed-phase HPLC (RP-HPLC ) and characterized by fast atom bombardment mass spectrometry (FAB-MS ) in order to ascribe mass and sequence (tandem MS) to peptides, Resul ts obtained confirmed that this HPSEC process involves mostly ion exch ange and size-exclusion. This HPSEC chromatographic procedure was foun d to be suitable for comparison of casein hydrolysates during producti on. (C) 1997 Elsevier Science B.V.