HPLC OF OLIGODEOXYRIBONUCLEOSIDE PHOSPHOR OTHIOATES

Authors
METELEV VG TASHLITSKII VN AGRAWAL S
Citation
Vg. Metelev et al., HPLC OF OLIGODEOXYRIBONUCLEOSIDE PHOSPHOR OTHIOATES, Bioorganiceskaa himia, 23(9), 1997, pp. 742-746
Citations number
11
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
23
Issue
9
Year of publication
1997
Pages
742 - 746
Database
ISI
SICI code
0132-3423(1997)23:9<742:HOOPO>2.0.ZU;2-Y
Abstract
Conditions are described for the separation of synthetic oligodeoxyrib onucleoside phosphorothioates by HPLC using a SCOUT WP PEI weak anion exchanger and for the separation of oligodeoxyribonucleotides with mix ed phosphate and phosphorothioate internucleotide bonds on a Partisphe re C-18 support in ion-pair conditions. The influence of the nucleosid e composition and the number of phosphorothioate bonds on the retentio n time was studied.