T. Anai et al., ISOLATION AND CHARACTERIZATION OF AN AUXIN-BINDING PROTEIN GENE FROM RADISH, AND ITS EXPRESSION IN INSECT CELLS, Physiologia Plantarum, 101(3), 1997, pp. 606-611
Auxin-binding protein (ABP1) is a putative receptor for auxin in the p
lasma membrane. We isolated a full-length cDNA encoding ABP1 from radi
sh by screening a cDNA library with its partial cDNA fragment generate
d by the reverse transcriptase-polymerase chain reaction (RT-PCR) meth
od. Radish abp1 mRNA was highly expressed in cotyledons, hooks and hyp
ocotyls, and less so in roots of radish seedlings. The deduced amino a
cid sequence of radish abp1 consisted of conserved auxin-binding motif
s, a signal peptide and ER-retention signal, and contained two potenti
ally N-linked glycosylation signals. To analyze the biochemical proper
ties of the radish abp1 product, this cDNA was expressed in insect cel
ls by the baculovirus vector system. The result of tunicamycin-treatme
nt showed that the signal peptide was cleaved and that the radish abp1
product was glycosylated at both target sites in insect cells.