INDIRECT TIME-RESOLVED FLUORESCENCE DETECTION OF BOTH NONFLUORESCENT AND FLUORESCENT COMPOUNDS SEPARATED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
KUROSU Y IWATA T TSUJI A MAEDA M
Citation
Y. Kurosu et al., INDIRECT TIME-RESOLVED FLUORESCENCE DETECTION OF BOTH NONFLUORESCENT AND FLUORESCENT COMPOUNDS SEPARATED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 787(1-2), 1997, pp. 261-265
Citations number
13
Journal title
Journal of chromatography → ACNP
Volume
787
Issue
1-2
Year of publication
1997
Pages
261 - 265
Database
ISI
SICI code
Abstract
Indirect time-resolved fluorescence (ITRF) detection of non-fluorescen t phenylthiohydantoin (PTH)-Ala and fluorescent dansyl (DNS)-Ala separ ated by high-performance liquid chromatography (HPLC) was carried out. Addition of europium (Eu3+) chelates to the eluent makes it possible to detect both non-fluorescent and fluorescent compounds simultaneousl y. This is because the fluorescence lifetime of Eu3+ chelates is extre mely long, whereas that of normal fluorescent compounds is short. The HPLC conditions with the Eu3+ chelate system were studied. (C) 1997 El sevier Science B.V.