EFFECTS OF REMOVING CIRCULATORY TUMOR-NECROSIS-FACTOR BY IMMUNOADSORPTION ON EXPERIMENTAL ENDOTOXIN-SHOCK ANIMALS

Objective To evaluate the effects of removing circulatory tumor necros is factor (TNF) by immunoadsorption on endotoxin shock animals. Method s Sixty New Zealand white rabbits were injected intravenously with let hal dose of endotoxin (10 Billion cfu/kg E. Coli endotoxin) and random ly divided into 3 groups: perfusion group, hemoperfusion started at 1 hour after injecting endotoxin through immunoadsorbent columns against TNF; pseudoperfusion group, hemoperfusion through blank columns; and control group, injected with endotoxin only. The arterial pressure, mi crocirculation of the mesentery, plasma levels of TNF, IL-1, IL-6, IL- 8, nitrite, endothelin-1 (ET-1), alanine aminotransferase (ALT), aspar tate aminotransferase (AST), creatinine were measured and analyzed and finally the survival rate was observed. Results Plasma levels of TNF were sharply reduced after immunoadsorption. Moreover, release of IL-1 , IL-6, IL-8, NO and ET-1 were also attenuated. Hemodynamic abnormalit ies could be improved and survival rate ameliorated significantly. Con clusion Specific immunoadsorption of circulating TNF might be a new an d effective therapy for endotoxin shock.