I. Blange et al., SPECIES-DIFFERENCES IN CARDIAC THYROID-HORMONE RECEPTOR ISOFORMS PROTEIN ABUNDANCE, Biological & pharmaceutical bulletin, 20(11), 1997, pp. 1123-1126
Little is known about the cardiac expression of different thyroid horm
one receptor (TR) isoforms. The aim of the study was to investigate su
ch patterns of TR expression at the protein level in different species
and in some human tissues. Western blot analysis with specific polycl
onal rabbit antibodies to each TR isoform was performed with samples f
rom myocardium of the Left ventricle from man, dog, guinea pig, rat an
d mouse, as well as with samples from several human tissues such as he
art, skeletal muscle, brain, liver and thyroid. The TR alpha 1 isoform
was present in all of the species examined. The TR alpha 2 was recogn
ized in human, dog and guinea pig heart, while no such band was recogn
ized in rat and mouse hearts. TR beta 1 was not detected in the human
heart but in the other species. Simularly to TR alpha 1, TR beta 2 was
detected in all of the species examined. In the human tissues studied
, TR alpha 1 was detected in heart and skeletal muscle, whereas TR alp
ha 2 was found only in the heart. TR beta 1 was not detected in any of
the examined human tissues, while TR beta 2 was found in all of them.
These results revealed unique distributions of TR variants and they d
emonstrate common epitopes in TR in the different species. For the fir
st time, the presence of a TR beta 2 isoform has been shown in human t
issues. TR isoforms may have a tissue and species specific role in the
regulation of gene expression and may in part explain variable tissue
effects of thyroid hormones.