COMPARISON OF BONE-MARROW TOXICITY OF MEDIUM-CHAIN AND LONG-CHAIN TRIGLYCERIDE EMULSIONS - AN IN-VITRO STUDY IN HUMANS

Background: In this study, we evaluated the in vitro bone marrow toxic ity of two lipid emulsions containing either long-chain triglycerides (LCT) or a mixture of medium-chain triglycerides (MCT) and LCT. Method s: Bone marrow cells were obtained from six healthy subjects and were cultured for 14 days after a 24-hour preincubation with various concen trations (from 0 to 10 mg/mL) of LCT-and LCT/MCT-based lipid emulsions . Results: Compared with controls (no preincubation with lipid emulsio n), both lipid emulsions significantly inhibited by 50% to 70% colony formation of all the human bone mac-row cells cultured from a triglyce ride concentration of 0.5 mg/mL (p < .05). Erythroid burst-fonuing uni t (BFU-E) formation was significantly more inhibited with LCT/MCT emul sion than with LCT emulsion (p < .05). The inhibition of granulocyte-m acrophage colony-forming unit (GM-CFU) and mixed granulocyte-erythrocy te-monocyte-megakaryocyte colony-forming unit (GEMM-CFU) formation did not significantly differ with the two emulsions. Conclusions: Both LC T-and LCT/MCT-based lipid emulsions strongly inhibit colony formation by human bone marrow cells. BFU-E colony formation is more sensitive t o LCT/MCT inhibition than to LCT.