INVOLVEMENTS OF TRP23 IN THE CHITIN-BINDING AND OF TRP131 IN THE CHITINASE ACTIVITY OF RYE SEED CHITINASE-A

The oxidation of the tryptophan residues of rye seed chitinase-a (RSC- a) and its isolated catalytic (Cat) domain by N-bromosuccinimide (NBS) was investigated in the absence and presence of oligomers of N-acetyl glucosamine (GlcNAc)(n). Based on the reactivity toward NBS at pH 5.9, the seven tryptophan residues present in RSC-a are grouped into highl y reactive (HR-), low reactive (LR-), and unreactive residues. Analyse s of the peptides from 1 tryptophan-and 3 tryptophan-oxidized RSC-a sh owed that the HR-residue is Trp23 and the LR-residues are Trp131 and T rp141. The chitin-binding ability of RSC-a was lost upon the NBS oxida tion of Trp23 at pH 5.9 or pH 7.0. This oxidation was prevented by (Gl cNAc)(3), which induced a high UV-difference spectrum with maxima at 2 84 and 293 nm. On the other hand, the chitinase activity of the Cat do main was greatly reduced by the NBS oxidation of Trp131 and Trp141 at pH 5.9, while in the NBS oxidation at pH 6.4, approximately one trypto phan residue was oxidized and about half of the activity was retained. The NBS oxidation of the isolated Cat domain at pH 5.9 was protected by (GlcNAc)(4), which induced a UV-difference spectrum with maxima at 284 nm and 293 nm as well as a small trough around 300 nm, similar to that observed in RSC-c. From these results and the previous result tha t Trp72 in RSC-e is involved in the substrate-binding, it was suggeste d that Trp23 is highly exposed on the surface of the RSC-a molecule an d involved in the chitin-binding, while Trp131 is involved in substrat e-binding in its enzyme action.