MANUFACTURING HIGH-PURITY MALTOSE AND MALTOTETRAOSE FROM STARCH BY A NOVEL AND EFFICIENT PROCEDURE NAMED REDUCING END MODIFICATION METHOD

A novel and efficient procedure named ''reducing end modification meth od'' was developed for manufacturing high purity maltooligosaccharides . By the method, high purity maltose and maltotetraose were prepared f rom starch. Starch was liquefied, debranched, and oxidized with sodium hypochlorite at the reducing end. The reaction mixture was treated wi th beta-amylase or maltotetraose-forming amylase from Pseudomonas stut zeri IFO-3773, resulting in the production of high purity maltose or m altotetraose, oxidized maltooligosaccharides, and oxidized undigested dextrin. High purity maltose (maltose content, 99.9%) or maltotetraose (maltotetraose content, 95%) was obtained very effectively from the r eaction mixture by chromatography on a strong acid cation exchange res in (Na+).