MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY OF MEMBRANE-PROTEINS - DEMONSTRATION OF A SIMPLE METHOD TO DETERMINE SUBUNIT MOLECULAR-WEIGHTS OF HYDROPHOBIC SUBUNITS

Authors
GHAIM JB TSATSOS PH KATSONOURI A MITCHELL DM SALCEDOHERNANDEZ R GENNIS RB
Citation
Jb. Ghaim et al., MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY OF MEMBRANE-PROTEINS - DEMONSTRATION OF A SIMPLE METHOD TO DETERMINE SUBUNIT MOLECULAR-WEIGHTS OF HYDROPHOBIC SUBUNITS, Biochimica et biophysica acta. Biomembranes, 1330(2), 1997, pp. 113-120
Citations number
27
Journal title
Biochimica et biophysica acta. Biomembranes → ACNP
ISSN journal
00052736
Volume
1330
Issue
2
Year of publication
1997
Pages
113 - 120
Database
ISI
SICI code
0005-2736(1997)1330:2<113:MLIMOM>2.0.ZU;2-E
Abstract
Matrix-assisted laser desorption ionization (MALDI) mass spectrometry has been used to obtain accurate molecular weight information for each subunit of several hydrophobic integral membrane proteins: cytochrome bo(3) (4 subunits) and cytochrome bd (2 subunits) from E. coli, and t he bc(1) complex (3 subunits) and the cytochrome c oxidase (3 subunits ) from Rhodobacter sphaeroides. The results demonstrate that the MALDI method is a convenient, quick, sensitive and reliable means for obtai ning the molecular masses of the subunits of purified multisubunit mem brane proteins. (C) 1997 Elsevier Science B.V.