APPLICATION OF O-HYDROXYPHENYLFLUORONE AS A FLUOROGENIC INDICATOR TO THE DETERMINATION OF HYDROGEN-PEROXIDE AND OXIDASE SUBSTRATES BASED ONTHE CATALYTIC EFFECT OF HEMIN

A highly sensitive fluorescence quenching method has been developed fo r selective determination of hydrogen peroxide based on the catalytic effect of hemin on the o-hydroxyphenylfluorone (a new fluorogenic subs trate) and hydrogen peroxide system. Under optimum conditions, the cal ibration graph was linear over the range 0-1.0 X 10(-6) mol/liter hydr ogen peroxide, with a limit of detection of 8.0 x 10(-9) mol/liter in a 10-min reaction period. It can easily be incorporated into the deter mination of biochemical substances that produce hydrogen peroxide unde r catalytic oxidation by their oxidase. This possibility has been test ed for the determination of glucose inhuman sera as an example. (C) 19 97 Academic Press.