P. Jagasia et al., ENHANCED PHOTOACTIVATED LUMINESCENCE OF SELECTED POLYCHLORINATED BIPHENYL CONGENERS AND AROCLOR MIXTURES, Microchemical journal, 57(3), 1997, pp. 350-360
Fluorescence studies of selected polychlorinated biphenyl (PCB) congen
ers and Aroclor mixtures were conducted using enhanced photoactivated
luminescence. Diphenylamine (DPA) was used as a photoactivator. DPA re
acts with PCBs to form a fluorescent photoproduct under UV activation.
The sensitivities of fluorescence detection using three different sol
id substrates were compared. Background studies of the substrates and
DPA blanks were also conducted to determine the substrate with the bes
t detection characteristics for enhanced photoactivated fluorescence.
It was found that the Envi disk with a glass-fiber mesh and C-18-modif
ied silica provides more analyte access for fluorescence detection, th
ereby providing best sensitivity. By comparison of the fluorescence fr
om pure PCB congener-DPA complexes, the more coplanar congeners exhibi
ted a red shift in excitation and emission as compared with the outho-
substituted nonplanar congeners. These studies may prove useful in det
ermining trends relating fluorescence intensity and toxicity factors.
(C) 1997 Academic Press.