PREPARATION OF CDNA-SILICA USING REVERSE-TRANSCRIPTASE AND ITS DNA-SEQUENCE DETERMINATION

A new method for producing macroporous silica (suitable for high-perfo rmance liquid chromatography) with covalently attached DNA is presente d. The method uses (dT)(18)-silica as a primer, annealed to a poly(A)- RNA template, which is then transcribed using reverse transcriptase. T he RNA template is eluted and single-stranded cDNA-silica is recovered . The cDNA-silica can be sequenced using the dideoxy method. These met hods provide a facile method for producing cDNA-silica of demonstrable authenticity and provide a unique approach to DNA and RNA sequencing.