DETERMINATION OF PLATINUM IN TUMOR-TISSUES AFTER CISPLATIN THERAPY BYELECTROTHERMAL ATOMIC-ABSORPTION SPECTROMETRY

A simple procedure is described for the determination of platinum in t umours after cisplatin therapy. Tumours were digested in 65% nitric ac id by incubation at 37 degrees C: for 2 days and platinum analysed und er optimum conditions by electrothermal atomic absorption spectrometry with Zeeman background correction. The reproducibility of measurement s in general was better than +/- 2%. The calibration graph nias linear from 30.0 up to 1000 mu g l(-1) of platinum, while the limit of detec tion (3 sigma) was found to be 3.0 mu g l(-1) (sample volume 20 mu). A queous standard solutions and the standard addition method were applie d ic, the calibration procedure. Under the recommended analytical cond itions, the sample matrix did not influence the determination of plati num significantly. In 72% of samples analysed the differences between results obtained by the two calibration procedures did not exceed +/- 5%. (C) 1997 Elsevier Science B.V.