ION-PAIR CHROMATOGRAPHIC METHOD FOR THE ANALYSIS OF MAFENIDE ACETATE

Mafenide acetate (Sulfamylon) is an antibacterial agent used to preven t and treat burn wound sepsis. No LC method is currently available for the analysis of this drug. A simple LC method was developed and valid ated for the analysis of sulfamylon. The chromatographic separation wa s achieved on a reversed-phase, C-18 column with UV detection at 270 n m. This isocratic system was operated at ambient temperature and requi red a B-min run-time. The mobile phase consisted of methanol-phosphate buffer pH 5.0 (65:35, v/v) and 2 mM 1-heptanesulfonic acid as the ion -pairing agent. The flow-rate was maintained at 1.0 ml/min. Standard c urves were linear over the concentration range of 2 to 100 mg l(-1). W ithin-day and day-to-day relative standard deviations were less than 2 .4% and 4.4%, respectively, This method was used to quantify mafenide acetate in pharmaceutical formulation.